A new simple, sensitive high performance thin layer chromatographic (HPTLC) method has been developed for the quantitative estimation of Levosulpride (LEVO) and Pantoprazole Sodium (PANTO) in combined dosage form using silica gel 60 F254 TLC plate using Toluene: Ethyl Acetate: Methanol: Acetic Acid (7:2:1:0.2 v/v/v/v) as mobile phase. LEVO and PANTO showed Rf value 0.23 + 0.005 and 0.40+0.006 and scanned at 288 nm using a camag TLC scanner 3. The method was validated in terms of linearity 75-420 ng/spot for LEVO and 40-240 ng/spot for LEVO .The limit of detection and limit ofquantification for LEVO and PANTO were found to be 12.625 ng/spot and 8.996 ng/spot, respectively and limit of quantification for LEVO and PANTO were found to be 38.259 ng/spot and 27.262 ng/spot, respectively. The mean recovery was 98.36-101.05% and 98.37-102.00% for LEVO and PANTO respectively. The method was found to be simple, sensitive, specific, accurate and precise and can be used for the routine quality control testing of Levosulpride and Pantoprazole Sodium dosage form. The results of analysis have been validated statistically as per ICH guidelines.
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